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In the dye reagent's recipe, I wonder how much is the total...

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Le Nghia Oct 19, 2016
protocol Protocol: Bradford Protein Assay
In the dye reagent's recipe, I wonder how much is the total volume of reagent. Is it about 150 ml, include 50 mg Coomassie G250, 50 ml Methanol, and 100 ml 85% Phosphoric acid? Don't we need to add mQ to adjust final volume?
And I think Methanol is poisonous, so can I replace it by Et Read more Read less more less
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Raj Aryan Kumar Answered Dec 7, 2017

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY

Hi! You can use Ethanol instead of Methanol.
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