I have the same questions, when I do stimulation of mouse splenocytes...

Protocol: Intracellular Cytokine (INF-gamma) Staining Assay

I have the same questions, when I do stimulation of mouse splenocytes with 500 ng/ml ionomycin, 500 ng/ml PMA and Golgistop at the same time fro about 4 hours, the cells looked like they all die or lyse. So I have to stop at this step. Did I add too much PMA? but according to BD protocol, Read more Read less

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