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A big doubt: Im using an Alkaline Phosphatase from Invitrogen,...
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Juan Carlos Catalan Aug 23, 2016
A big doubt: Im using an Alkaline Phosphatase from Invitrogen, this enzyme has its own buffer dilution, should i using a dilution?
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Fanglian He Author Answered Aug 24, 2016
Bio-protocol
Hi Juan,
As said in the protocol, "normally NEB digestion buffer is good for CIP enzyme", so, in my case, I did not use the buffer that comes with CIP enzyme. But, I did not use Alkaline Phosphatase from Invitrogen. So, you may need to check if your digestion buffer is also good for your Alkaline Phosphatase.
Hope this helps.
--Fanglian
As said in the protocol, "normally NEB digestion buffer is good for CIP enzyme", so, in my case, I did not use the buffer that comes with CIP enzyme. But, I did not use Alkaline Phosphatase from Invitrogen. So, you may need to check if your digestion buffer is also good for your Alkaline Phosphatase.
Hope this helps.
--Fanglian
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