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Hai. I want to ask, how many the volume of RNase should i added...

ku dyyla Aug 11, 2016

Protocol: E. coli Genomic DNA Extraction

Hai. I want to ask, how many the volume of RNase should i added into my lysis buffer. Because i want to remove the RNA.
Sorry i can understand the explaination in the note on step no8

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Dileep Kumar Answered Feb 26, 2017

Institute of biotechnology and genetic engineering,university of sindh,jamshoro,Pakistan

Thanks Fanglin

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Fanglian He Author Answered Aug 11, 2016

Bio-protocol

Hi Ku,

Depending on the concentration of your RNAase stock, a final concentration of RNAase in your lysis buffer is about 1 mg/ml.

Hope this helps.

Good luck,
Fanglian

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