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Hello. I didn't understand step 6 (gel purification). My other...
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Bioseeker B Jul 29, 2016
Hello. I didn't understand step 6 (gel purification). My other question is that how we should know how many microliters we should add in any step of any molecular based experiment? Should we always have a protocol? What if the amounts differ by protocols?
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Fanglian He Author Answered Aug 1, 2016
Bio-protocol
Hi, Regarding gel purification, any commercial kit of gel purification (i.e.
QIAquick Gel Extraction Kit ) can be used.
For your second question, not sure what you meant by "any molecular based experiment". But, the amount of each reagent presented in this protocol worked in my hand. Of course, you could adjust the amount of each reagent accordingly if the volume of your reaction assay is different.
QIAquick Gel Extraction Kit ) can be used.
For your second question, not sure what you meant by "any molecular based experiment". But, the amount of each reagent presented in this protocol worked in my hand. Of course, you could adjust the amount of each reagent accordingly if the volume of your reaction assay is different.
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