I have 2 questions please1. What are the appropriate conditions...

GM
Gugu Musa
May 10, 2016
protocol Protocol: Isolation of Genomic DNA from Mycobacterium Species
I have 2 questions please
1. What are the appropriate conditions to lyse Mycobacterium smegmatis using sonication method (time, temperature, amplitude and others...)
2. Do I have to use Urea as part of my lysis buffer? If yes, at what concentration?
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