How do you obtain the volume of bacterial solution needed to...

Answer: At first, the amount of bacteria in 1 OD600 (checked with a spectrophotometer) solution was determined. To get the result, 0.1 mL 1 OD600 bacterial solution was diluted with 10-time serial dilution for 6-7 times. Then the serial diluted bacterial solutions were inoculated and the colonies were measured after overnight culture. Next, the original amount of bacteria in these diluted solutions could be deduced by the colony number and dilution fold. Finally, we found that 1 OD600= 10*9 CFU/ml. Therefore, 0.01 OD600 = 10*7 CFU/ml.