I wish to evaluate the effects of a neurotoxin on C. elegans. The exposure time will be over a 24 h period. Would you recommend exposing the worms to the S-buffer as the control and the treatment solutions in various concentrations of toxin in S-Buffer?
Department of Biology, The Pennsylvania State University, USA
I do not really know the answer to your question. You might try the neurotoxin in M9 as well, just in case that cholesterol in the S-basal buffer may affect neurotoxin's effects.