Improve Research Reproducibility A Bio-protocol resource

i've been performing the intratracheal instillation in mice...

SL
Spencer Li Feb 27, 2016
protocol Protocol: Non-invasive Intratracheal Instillation in Mice
i've been performing the intratracheal instillation in mice with your methods. After instillation, the mouse starts coughing which may cause the inconsistent doses of LPS.
how to avoid the coughing during the procedure???
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

Mark R. Looney Author Answered Feb 29, 2016

Department of Medicine, University of California, San Francisco, USA

There will always be some respiratory distress after an intratracheal liquid challenge--that cannot be avoided. I would keep the instillate volume to less than 50 microliters if at all possible. Also, deeper sedation usually helps blunt the coughing response.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer