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Dear Dr. Gupta,I plan to use your protocol for my experiment....
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Busra Yagabasan Feb 24, 2016
Dear Dr. Gupta,
I plan to use your protocol for my experiment. But I have 2 questions. I would be grateful if you can help me about them.
The first question is: Do you starve the mice including also food overnight?
Second question is: After the oral gavage, Read more
I plan to use your protocol for my experiment. But I have 2 questions. I would be grateful if you can help me about them.
The first question is: Do you starve the mice including also food overnight?
Second question is: After the oral gavage, Read more
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LI YEQIONG Answered Mar 29, 2016
NANJING UNIVERSITY
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0 comments ShareAngel R. Nebreda Author Answered Feb 25, 2016
Institute for Research in Biomedicine (IRB Barcelona), Spain
Dear Bursa,
Thank you very much for your interest in our protocol.
Yes, we only water starve the mice as also mentioned in the protocol. In my recent experience, 5-6 h of water starvation also reduces variability between mice.
Regarding your second query, during the 4h waiting time we put the water bottle back.
I hope this information will help you to design your experiment.
Best,
Jalaj Gupta
Thank you very much for your interest in our protocol.
Yes, we only water starve the mice as also mentioned in the protocol. In my recent experience, 5-6 h of water starvation also reduces variability between mice.
Regarding your second query, during the 4h waiting time we put the water bottle back.
I hope this information will help you to design your experiment.
Best,
Jalaj Gupta
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