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Dear sir/madame,I've followed the exact protocol to find sulfated...
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Mikhail Olinevich Feb 23, 2016
Dear sir/madame,
I've followed the exact protocol to find sulfated glycosaminoglycans in human skin samples which have been degraded using pronase. A kit from "Biocolour, Blyscan" quantifies the sGAGs in our sample in a proper way. Because of its expensiveness, we have decided t
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I've followed the exact protocol to find sulfated glycosaminoglycans in human skin samples which have been degraded using pronase. A kit from "Biocolour, Blyscan" quantifies the sGAGs in our sample in a proper way. Because of its expensiveness, we have decided t
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Vivien J. Coulson-Thomas Author Answered Mar 2, 2016
University of Houston
Dear Mikhail,
I am not sure if I understood the extent of the problem with the standard curve. Were 3, 4 and 5 ng shown in your photo done with the reagent prepared based on our protocol? They seem to have worked. They are very close points on the curve so maybe you could open the curve a little. Use 1.25 microg/mL, 5 microg/mL and 10 microg/mL while testing.
Now about your samples, what are they and what were they prepared in? Some detergents or high salts like NaCl and/or Urea can affect the DMB reaction. Could you please provide more information on how these samples were purified?
Thank you.
I am not sure if I understood the extent of the problem with the standard curve. Were 3, 4 and 5 ng shown in your photo done with the reagent prepared based on our protocol? They seem to have worked. They are very close points on the curve so maybe you could open the curve a little. Use 1.25 microg/mL, 5 microg/mL and 10 microg/mL while testing.
Now about your samples, what are they and what were they prepared in? Some detergents or high salts like NaCl and/or Urea can affect the DMB reaction. Could you please provide more information on how these samples were purified?
Thank you.
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