I am a Biology Research Student working on lifespan research....

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Mike Morris
Feb 16, 2016
protocol Protocol: Common Worm Media and Buffers
I am a Biology Research Student working on lifespan research. I am using FuDR and it appears toxic during research experiments. Do you have any advice in reference to using FuDR? I am applying the FuDR in with the agar after it cools to an appropriate temperature. Thanks
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Evrim Çelebi Answered Dec 18, 2016

Ankara university

Hello,

Mr. Peichuan ı would to know any alternative for plate which seeded FuDR such as liquid culture. if answer is yes how can I perform with liquid culture for lifespan experiment?
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Peichuan Zhang Answered Apr 12, 2016

Department of Biology, The Pennsylvania State University, USA

It has been shown that FuDR by itself can have effects on lifespan. For example, a recent work by Anne Hart and co-workers has shown that FuDR induces stress response and it, in combination with hypertonic stress, can extend the lifespan of wild-type C. elegans by 30%.
http://www.ncbi.nlm.nih.gov/pubmed/?term=26854551

The Hekimi lab also showed that FuDR can extend the lifespan of gas-1 mitochondrial mutants, which are relatively short-lived, by ~2-fold.
http://www.ncbi.nlm.nih.gov/pubmed/?term=21893079

In this regard, I will recommend using FuDR at the final concentration of ~25uM to 40uM. It'd be better to add FuDR (diluted in 500ul M9) directly onto the seeded lawn of bacteria and wait for at least one day before using the plate for any experiment.
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