Peichuan Zhang Answered Apr 12, 2016
Department of Biology, The Pennsylvania State University, USA
It has been shown that FuDR by itself can have effects on lifespan. For example, a recent work by Anne Hart and co-workers has shown that FuDR induces stress response and it, in combination with hypertonic stress, can extend the lifespan of wild-type C. elegans by 30%.
http://www.ncbi.nlm.nih.gov/pubmed/?term=26854551
The Hekimi lab also showed that FuDR can extend the lifespan of gas-1 mitochondrial mutants, which are relatively short-lived, by ~2-fold.
http://www.ncbi.nlm.nih.gov/pubmed/?term=21893079
In this regard, I will recommend using FuDR at the final concentration of ~25uM to 40uM. It'd be better to add FuDR (diluted in 500ul M9) directly onto the seeded lawn of bacteria and wait for at least one day before using the plate for any experiment.