In my experiment, I found some different result. In gel electrophoresis, there is thick band in lower part in agarose gel (Fig 1). In protocol, there is just a DNA band in upper part... And also, that lower band is not disappear after MNase treatment (FIg 2). I could seRead moreRead less
Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Spain
Dear Hyub-Bi,
In our hands we never observe this strong low molecular band you are observing in your samples. I am afraid it might be RNA contamination. Maybe, you could do a RNAse treatment at the end of steps 14 or 15.