In my experiment, I found some different result.In gel...

HK
Hyub-Bi Kim
Feb 1, 2016
protocol Protocol: Micrococcal Nuclease (MNase) Assay of Arabidopsis thaliana Nuclei
In my experiment, I found some different result.
In gel electrophoresis, there is thick band in lower part in agarose gel (Fig 1).
In protocol, there is just a DNA band in upper part...
And also, that lower band is not disappear after MNase treatment (FIg 2).
I could se
img Read more Read less more less
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

Jordi Moreno-Romero Author Answered Feb 1, 2016

Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Spain

Dear Hyub-Bi,

In our hands we never observe this strong low molecular band you are observing in your samples. I am afraid it might be RNA contamination. Maybe, you could do a RNAse treatment at the end of steps 14 or 15.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer