Dear Nabila, I have a question about you protocol, why you...

guo dianlei
guo dianlei
Dec 17, 2015
protocol Protocol: Lentivirus Production
Dear Nabila,
I have a question about you protocol, why you used the DMEM solution containing 30% FBS as the harvest media, in my view, DMEM containing 10% FBS or no serum media used widely in my lab
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Nabila Aboulaich Author Answered Dec 17, 2015

Like for cells, FBS serves as a stabilizer during long term storage of virus. The bovine serum albumin in FBS preserves the virus by preventing ice Krystal formation. This reduces loss of virus infectivity after freeze and thaw cycles. Other stabilizers can be used, such as sucrose.
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