Dear Nabila, I have a question about you protocol, why you...
Dear Nabila,
I have a question about you protocol, why you used the DMEM solution containing 30% FBS as the harvest media, in my view, DMEM containing 10% FBS or no serum media used widely in my lab
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Like for cells, FBS serves as a stabilizer during long term storage of virus. The bovine serum albumin in FBS preserves the virus by preventing ice Krystal formation. This reduces loss of virus infectivity after freeze and thaw cycles. Other stabilizers can be used, such as sucrose.
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