Hi, I'm trying to set up your protocol and I wanted to confirm...

RH
Rodrigo Hoyos
Dec 3, 2015
protocol Protocol: Flow Cytometric Detection of Reactive Oxygen Species
Hi, I'm trying to set up your protocol and I wanted to confirm if NAC/H2O2 were present in the cell culture media while the cells were getting to a confluency of 70-90% (for positive and negative controls respectively) and after cells were treated for an adittional hour. (Steps 2, 3 and 4 Read more Read less more less
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Rodrigo Hoyos Answered Dec 4, 2015

Boston Children's Hospital

Thanks a lot for your answer, that's what i figured but I got confused since in step two it says "Cells were treated with NAC or H2O2 until 70-90% confluency reached" instead of after reaching 70-90% confluency.
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Hsin-Yi Chang Author Answered Dec 4, 2015

National Defense Medical Center

Hi, I'm not sure what your question is. According to the protocol, cells were treated with NAM or H2O2 after reaching 79-90% confluency. At the end of treatment, cells were trypsinized and incubated with dye containing DPBS.
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