Hi, Thanks for the detailed protocol. It seems like the Treg...

LQ
Lisa Qin
Sep 17, 2015
protocol Protocol: In vitro Regulatory T cells Differentiation From Naïve T Cells
Hi, Thanks for the detailed protocol. It seems like the Treg differentiation is carried out in serum free medium. Is it OK for T cells? Thanks.
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

Marian Salatino Author Answered Sep 22, 2015

Immunopathology Department, Institute of Biology and Experimental Medicine, CONICET, Argentina

That is correct! This protocol is carried out in serum free medium. Treg differentiation can be carried out like this but it is NOT OK for effector T cells as they will die unless serum is present. You can also differentiate Tregs in medium with serum. We have done it in 2%FCS containing medium and has given us great results. The proteins present in the serum can however retain TGFb and can thus impact negatively on the differentiation efficiency.
Write if you have any other doubts!
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer