Improve Research Reproducibility A Bio-protocol resource

Hello，I have two questions. Do I need to mixing the lysate adequately...

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Tao Zhang Sep 2, 2015
protocol Protocol: Analysis of Protein Stability by the Cycloheximide Chase Assay
Hello，I have two questions. Do I need to mixing the lysate adequately before transfer it to -80? How could I determine the concentration of CHX when I use the cells of rat embryonic fibroblast. Should I treated the cells with CHX with different concentrations for 8 hours and observe the ap Read more Read less more less
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