Can I use 2mm cuvvette for high competency method? What settings...

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raissa fedora
Aug 31, 2015
protocol Protocol: Burkholderia glumae Competent Cells Preparation and Transformation
Can I use 2mm cuvvette for high competency method? What settings should I use?
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raissa fedora Answered Aug 31, 2015

atma jaya catholic university

I couldn't replicate the method using my Burkholderia isolate. The electroporator says arc detected, meanwhile the conventional method works fine. I wonder what's the possibility of failure during pulsing..
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Hiromasa Saitoh Author Answered Aug 31, 2015

Tokyo University of Agriculture

We haven't used 2mm cuvette for high competency method. But you can try it according to the setting written in conventional method.
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