HELLO,could you suggest a protocol for S.aureus genomic DNA...

Hi Karthikeyan,

Sorry, I 've never done that experiment. Please use Bio-protocol RaP service (http://www.bio-protocol.org/RaP.aspx) to request such a protocol.

Thanks,
Fanglian

How can isolate The DNA from onions. Please help to me

thanks Fanglian:)

Hi, Humera,

Regarding the DNA ladder, please contact the company via the provided link above. Regarding your 2nd question, unfortunately, I did not record the time when I ran my genomic DNA from E coli. But, my impression was that it should not take too long. In case your sample was too condensed, you might run your samples with 1 or 2 more dilutions.

--Fanglian

hi ,
which DNA ladder was run in above image with genomic DNA?
how much time is required to migrate the genomic DNA at least from the well?

Hi,

It may simply diffuse, which is normal. Keep the tube at -20 degree for at least 30 min (the longer the better) and then spin it down. You should see DNA pellet (it looks transparency when it is wet, and turns to white when it becomes dry) .
--Fanglian

hello,
thankyou very much for the reference..i also had one doubt when we precipitate DNA with cold ethanol i see very nice precipitate as soon as etanol is added but which disappears on shaking or even slight mixing..what is the probable reason??

Hi, Upasana,

I have asked Prof. Chikara Kaito at the University of Tokyo for help with your question. Below is his response:

"The extracting protocol of S. aureus genomic DNA has been written in Novick RP, Methods in Enzymology, Vol 204, 587-636.
The method is written on page 589-590.

For convenience, we are usually using the extraction Kit from Qiagen (QIAamp DNA Blood Mini Kit, Cat No. 51106) after lysing S. aureus cells with lysostaphin."

Hope this would be helpful. Here, I want to thank Prof. Chikara Kaito again for his prompt response and helpful information.

Good luck,

--Fanglian

hey,thankyou I will be waiting

Hi,

I will contact one of our authors who works on S. aureus about your request and let you know when I receive his response.

--Fanglian