Hey, i've been searching for a solid genomic DNA extraction...

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Rachel Williams
Jul 23, 2015
protocol Protocol: E. coli Genomic DNA Extraction
Hey, i've been searching for a solid genomic DNA extraction protocol to follow for an transformed E.coli culture. The main difference i've noticed is the call for CTAB. Clearly the process can work without but do you know how beneficial it is to use? Thanks!
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Fanglian He Author Answered Jul 31, 2015

Bio-protocol

Thank you for sharing, Rachel!
From the original paper about CTAB method by Kate Wilson (http://onlinelibrary.wiley.com/doi/10.1002/0471142727.mb0204s56/abstract), it seems that CTAB can help to remove polysaccharide contamination in DNA prep. This would be useful since "exopolysaccharides can interfere with the activity of enzymes such as restriction endonucleases and ligases." (quoted from the original paper).
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Rachel Williams Answered Jul 24, 2015

University of southampton

hexadecyltrimethyl ammonium bromide
I've seen it in a few protocols such as this one:
http://1ofdmq2n8tc36m6i46scovo2e.wpengine.netdna-cdn.com/wp-content/uploads/2014/02/JGI-Bacterial-DNA-isolation-CTAB-Protocol-2012.pdf
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Fanglian He Author Answered Jul 23, 2015

Bio-protocol

Hi Rachel,

What does CTAB stand for? Any references?

Thanks,
Fanglian

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