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Hi, I would like to know that for how many days plate coated...

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Muzamil Want Jul 17, 2015
protocol Protocol: In vitro Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
Hi,

I would like to know that for how many days plate coated with antCD 3 and antiCD 28 is stable at 4 C before use?


Thanks

Muzamil
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Muzamil Want Answered Jul 19, 2015

THSTI

thanks Jia. I always coat the plate one day before but due to some problem in sorting of cells next day i could not plate cells. Thats why i asked for how many days i can keep the coated plate at 4 C.
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Jia Li Author Answered Jul 17, 2015

SRI International

Coat with aCD3 one day before experiment and keep at 4 degree
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