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Reading your protocol with great interest. Since we will conduct...

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Dong-Ming Su Jun 30, 2015
protocol Protocol: In vitro Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
Reading your protocol with great interest. Since we will conduct this experiment, I have a question: It looks that you do not need APCs (antigen presenting cells) in your culture system, why? What is difference with or without APCs? Thanks!
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Jia Li Author Answered Jun 30, 2015

SRI International

The differentiation do not need peptide presented by APCs, and anti-CD3/CD28 can activate T cells. If you want to check the differentiation of peptide-specific T cell, you may need APCs instead of anti-CD3/CD28.
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