why Glucose/Sucrose is added during plasmid DNA isolation and...

protocol Protocol: E. coli Genomic DNA Extraction
why Glucose/Sucrose is added during plasmid DNA isolation and not during genomic DNA?
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Pushpalatha.R pushpa Answered Jun 20, 2015

GKVK

Even I tv not tried so. In reference point of view. Few protocols shows use of glucose/sucrose for plasmid DNA extraction and not been used during genomic DNA isolation of a bacteria. Why so...
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Fanglian He Author Answered Jun 20, 2015

Bio-protocol

Hi,

For the protocol "Plasmid DNA Extraction from E. coli Using Alkaline Lysis Method", Glucose is added (in resuspension solution) to make the solution isotonic. In fact, isotonicity is not required for bacteria with cell walls (i.e. E. coli) because bacteria with cell walls can withstand wide range of solution concentration. Thus, glucose may not have to be included in the resuspension buffer. I never tried the one without glucose, though. So, please share your experience with us if you try it without glucose.

Good luck,
Fanglian
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