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Dear Fang,I tested the protocol and had my nuclei extraction...

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Ahmet B Apr 28, 2015
protocol Protocol: Nuclear Extraction from Arabidopsis thaliana
Dear Fang,

I tested the protocol and had my nuclei extraction run through mass spectrometry after my nuclear protein IP. but when I checked results , I realized the chloroplast contamination. there were high abundance of chloroplast proteins.
Do you have any suggestions to Read more Read less more less
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Fang Xu Author Answered Apr 28, 2015

Shandong University

Yes, you can add a centrifugation step after filtering (100g for 10 min). You can also wash the nuclear with one more time and make sure that the nuclear pellet has no green color. When you wash the the pellet,try to pipette enough to complete suspend the pellet. I hope those suggestions will help.
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