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If I want to extract FAME from small volume of cell culture...

kJ
kamran Jawed Apr 11, 2015
protocol Protocol: Quantification of Bacterial Fatty Acids by Extraction and Methylation
If I want to extract FAME from small volume of cell culture (100-500 ul), how can I modify this protocol.
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Bio-protocol Editorial Team Answered May 20, 2015

bio-protocol.org

Authors indicated that they did not try to use that small volume of cell culture for this protocol, thus they did not have good answer to your question.
Please share your experience with other readers via our "Reproducibility Feedback" if you successfully modify this protocol for that smaller vol. of cell culture in the future.

Good luck,

Bio-protocol team
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