Thanks for the detailed protocol. I would like to know whether...

protocol Protocol: Determination of Ferric Chelate Reductase Activity in the Arabidopsis thaliana Root
Thanks for the detailed protocol. I would like to know whether do you cut out shoot parts before taking fresh weight or do you use whole seedlings for assay?
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Santosh Satbhai Answered Apr 2, 2015

GMI-Vienna

Thank you.
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EMRE EMRE AKSOY Author Answered Apr 2, 2015

Agricultural Genetic Engineering, Nigde Omer Halisdemir University

Shoots are separated from the roots by sharp scissors before taking the fresh weight. Make sure your assay solution is ready waiting in the tubes before you do all cutting and transfer of roots so that the roots do not dry out.
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