Can you do a western on BMMC using anti FCεR1α to prove that...

BS
Barbara Summers
Mar 31, 2015
protocol Protocol: Isolation and Culture of Bone Marrow-derived Mast Cells
Can you do a western on BMMC using anti FCεR1α to prove that your cells are mast cells or is FACS only possible with this antibody.
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Krisztina V. Vukman Author Answered Apr 6, 2015

Dublin City University

You can do a western, but I think with that method you can only prove that you have mast cells in your cell culture, but are not able to check purity. I think Kimura stain is the easiest way to check purity. I ususally use facs, because it is very specific and reliable.
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