is it possible to freeze the PBMC and what is the best media...

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Shadia Nada
Mar 24, 2015
protocol Protocol: In vitro Culture of Human PBMCs
is it possible to freeze the PBMC and what is the best media to be use for freezing
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Santosh K Panda Author Answered Mar 26, 2015

Infectious Disease Biology, Institute of Life Sciences, India

We never tried in this way. If I have to advice you based on my experience it is not going to function physiologically equivalent to fresh cells. You can freeze them for DNA isolation or cell lysate preparation but not for any immune response study.
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Shadia Nada Answered Mar 26, 2015

University of Toledo

I’m purifying the PBMC from human blood and I use the cells right away I’d like to ask if I can freeze the rest of the cells for next experiment, and if I froze them, are they going to be function as the fresh cells if I thaw and use them again and what is the best media for freezing. Thank you for your help and time
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Santosh K Panda Author Answered Mar 26, 2015

Infectious Disease Biology, Institute of Life Sciences, India

Dear Shadia, Can you please clarify why you want to freeze??
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