is it possible to freeze the PBMC and what is the best media...
is it possible to freeze the PBMC and what is the best media to be use for freezing
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Santosh K Panda Author Answered Mar 26, 2015
Infectious Disease Biology, Institute of Life Sciences, India
We never tried in this way. If I have to advice you based on my experience it is not going to function physiologically equivalent to fresh cells. You can freeze them for DNA isolation or cell lysate preparation but not for any immune response study.
I’m purifying the PBMC from human blood and I use the cells right away I’d like to ask if I can freeze the rest of the cells for next experiment, and if I froze them, are they going to be function as the fresh cells if I thaw and use them again and what is the best media for freezing. Thank you for your help and time
Santosh K Panda Author Answered Mar 26, 2015
Infectious Disease Biology, Institute of Life Sciences, India
Dear Shadia, Can you please clarify why you want to freeze??
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