Improve Research Reproducibility A Bio-protocol resource

Dear all,I have a question: in what fraction of a sucrose gradient...

AF
Anna Feldman Mar 8, 2015
protocol Protocol: Ribosome Fractionation in Yeast
Dear all,
I have a question: in what fraction of a sucrose gradient (i mean, a percent) I will be able to detect a monosomes?
Many thanks,
Anna
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
4 answers
Sort by: most helpful / most recent

Anna Feldman Answered Mar 9, 2015

Tel Aviv University

Well, but I, simply, write some article and I need the reviewer.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

Olesya O. Panasenko Author Answered Mar 9, 2015

Department of Microbiology and Molecular Medicine, University of Geneva, Faculty of Medicine, Switzerland

I just calculated it by myself :)
You know the length of the gradient - 8.5 cm for 7-47% gradient. The peak of 80S comes exactly in the middle - at 4.2 cm. Gradient is linear. Thus - (7+47)/2 = 27%
0 helpful
0 unhelpful
comment 0 comments down up
share Share

Anna Feldman Answered Mar 9, 2015

Tel Aviv University

Dear Olesya,
Thanks for your cooperation. May you, please send me a some reference(article) link?
All the best,
Anna
0 helpful
0 unhelpful
comment 0 comments down up
share Share

Olesya O. Panasenko Author Answered Mar 9, 2015

Department of Microbiology and Molecular Medicine, University of Geneva, Faculty of Medicine, Switzerland

Dear Anne,
Peak of monosomes (80S) appears in the middle of 7-47% gradient (if you use my conditions), approximately at 27% of sucrose.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer