HongLok Lung Author Answered Mar 9, 2015
University of Hong Kong
Thanks for your message, Andres. How many cells did you inject?
Make sure you see the tumor cells bubble after injection, if the bubble is not formed, it is most likely that the implant will disappear. Similarily, if there is no tumor cells or not enough cells in the gel plug, the impalnt will be absorbed. You can try increasing the volume of matrigel (eg. up to 450ul gel and 50ul cells) or increasing the cell numbers (up to 1 x 10e7).
For the case of haematoma, have you done the tissue sectioning of the "haematoma" and subsequent H&E/CD34 staining? that happens sometimes since the tumor blood vessels are leaky.
Good Luck!