In some cases the whole implante becomes in a haematoma and...

AV
Andrés Vargas
Mar 7, 2015
protocol Protocol: In vivo Matrigel Plug Angiogenesis Assay
In some cases the whole implante becomes in a haematoma and in other cases the implant disappear around the fourth day post injection... some idea of what i am doing wrong?
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

HongLok Lung Author Answered Mar 9, 2015

University of Hong Kong

Thanks for your message, Andres. How many cells did you inject?

Make sure you see the tumor cells bubble after injection, if the bubble is not formed, it is most likely that the implant will disappear. Similarily, if there is no tumor cells or not enough cells in the gel plug, the impalnt will be absorbed. You can try increasing the volume of matrigel (eg. up to 450ul gel and 50ul cells) or increasing the cell numbers (up to 1 x 10e7).

For the case of haematoma, have you done the tissue sectioning of the "haematoma" and subsequent H&E/CD34 staining? that happens sometimes since the tumor blood vessels are leaky.

Good Luck!
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer