Improve Research Reproducibility A Bio-protocol resource

Hi, thank you for your protocol. Now I'll try to do the same...

zheng PM Feb 3, 2015

Protocol: Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)

Hi, thank you for your protocol. Now I'll try to do the same exp, and I have some questions.First, how did you distinguish the MSCs(marrow mesenchymal stem cells)and hematopoietic stem cell, due to the MSCs also easy to adhere to the plastic dish.If the MSCs can also be induced to Macropha Read more more

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Ran Chen Author Answered Mar 3, 2015

Zhejiang JFK Biological Technology Co. Ltd.

1. You don't need to distinguish the MSCs and the hematopoietic stem cells because only the hemnatopoietic stem cells can be induced by the L-conditioned medium which contains M-CSF and then attached to the plastic firmly. All other cells be lost during the medium-changing/washing process.
2. The scrape won't hurt the cells. The cells can be cultured and used for at least two weeks.

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