Dear all,i'm a post doc of bologna university. Recently we bought...

protocol Protocol: Polysome Profiling Analysis
Dear all,
i'm a post doc of bologna university. Recently we bought a teledyne isco gradient system, provided with UA6 UV detector, optical unit, and a fraction collector for polysome profile analysis. In your protocols you indicate the tracerdaq as the software used for computetional
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Masahiro Morita Author Answered Jan 28, 2015

University of Texas Health Science Center at San Antonio

Dear Carmine,

I would like to send the photos of UA6 detector and USB I/O adaptor to you. Would it be possible to let me know your email address?

All the best,

Masahiro Morita
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Carmine Onofrillo Answered Jan 27, 2015

Bologna University

Dear dott. Morita,
thanks for your fast reply. Actually i'm not sure about the connection of the cable from recorder output of UA6 he UA6 manual, detector to my device . Our device (as yours) can operate in single ended mode ( only +/- 10V) or in differential mode (+/- 10, 5, 2.5 ,2, 1.25, 1V), in single ended i have to use only 2 cable (POSITIVE and GROUND), in differential i have to connect 3 cables ( HI, LOW and GROUND). Do you know what settings i have to use? In The tracerdaq software and in instacal it is possible to check the type of connection (single ended or differential), in the settings menu. I hope you can help me.
Best regards,
Carmine Onofrillo.

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Ivan Topisirovic Author Answered Jan 26, 2015

Department of Oncology and Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, McGill University, Canada

Dear Carmine,

In our lab, USB-1208L is used to connect the UA6 detector to the laboratory computer. To connect the UA6 detector to USB digital I/O, this protocol may be of use (http://www.isco.com/pcfiles/PartPDF/SL000004/UP000ZUZ.pdf). Moreover, TracerDAQ software is included in USB-1208L with the protocol about the connection (http://www.microdaq.com/measurement_computing/usb_data_acquisition/multifunction/usb-1208/usb-1208ls-daq.php). If you need any further information, please do not hesitate to contact us.

All the best,

Masahiro Morita
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