necesito determinar cuanto de proteína tengo en mi muestra;cuanta...

Hi, Maria,

My apologies for the late response. To determine how much sample is used for BCA protein assay, you should first make several dilutions of your sample, like 1:10, 1:20, 1:50... (if your sample is limited, you can start with a higher dilution. The absorbance readings (at 562 nm) which fall into the linear section of the standard curve can be used to calculate the concentration of your undiluted protein sample.

Hope my answer helps. Please let me know if anything is unclear to you.

Good luck,
Fanglian

I need to determine how much protein I have in my sample;
How much sample need to use this method or as both of my sample used in this method?

I'm not good in english sorry, but is important for me know that both of my sample can process this method if I have only 1mg can used this method.

I explain?

Hi Maria,

Would you lease put your question in English? Thanks.