Tsung-Luo Jinn Author Answered Jan 4, 2015
Department of Life Science and Institute of Plant Biology, National Taiwan University
Hi,
Please extract your sample with ice-cold buffer, and then run your gel in cold room,
Which can give you best result,
Sure, SOD may or may not denature when temp higher then 4C, different SOD from different sample do have it optimal reaction temp and pH value,
Best,