DOES SOD GETS DENATURED AT TEMPERATURES HIGHER THAN 4 DEGREE...

Rashmi Mishra
Rashmi Mishra
Jan 1, 2015
protocol Protocol: Cellular Extract Preparation for Superoxide Dismutase (SOD) Activity Assay
DOES SOD GETS DENATURED AT TEMPERATURES HIGHER THAN 4 DEGREE CELCIUS?
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Tsung-Luo Jinn Author Answered Jan 4, 2015

Department of Life Science and Institute of Plant Biology, National Taiwan University

Hi,
Please extract your sample with ice-cold buffer, and then run your gel in cold room,
Which can give you best result,
Sure, SOD may or may not denature when temp higher then 4C, different SOD from different sample do have it optimal reaction temp and pH value,
Best,
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