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On step 15 it says to discard non-adherent cells. Did you mean...

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Barbara Summers Dec 11, 2014
protocol Protocol: Isolation and Culture of Peritoneal Cell-derived Mast Cells
On step 15 it says to discard non-adherent cells. Did you mean to spin down media and resuspend non adherent cells in fresh media and then plate into a new flasks?

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Barbara
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Krisztina V. Vukman Author Answered Oct 1, 2018

Dublin City University

Yes
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