Is the cell in growth status or starvation status, means the...

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Carl Lee
Dec 1, 2014
protocol Protocol: Scratch Wound Healing Assay
Is the cell in growth status or starvation status, means the concentration of FBS should be 10% or 0.1%-1%, when the cell treated with drugs? Thank you.
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Yanling Chen Author Answered Dec 2, 2014

The Scripps Research Institute

The protocol described here is for what you called "growth status", and you can modify it according your experimental requirements-it really depends on your assay goals and cell type. As you probably know, many cells (even cancer cells) in low serum media won't grow as well as in high (i.e., 10%) serum conditions. If your purpose is to maximize the effect of a drug under low serum conditions, you can try your cells of interest at lower FBS for sure, but the assay conditions should be empirically determined (for a better "combination" of assay settings).
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