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how to use Alexa Fluor 488,?what is dilution and how to dilute...

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garima tripathi Nov 18, 2014
protocol Protocol: Phalloidin Staining and Immunohistochemistry of Zebrafish Embryos
how to use Alexa Fluor 488,?
what is dilution and how to dilute for ready to use?
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Michelle F Goody Answered Nov 21, 2014

Graduate School of Biomedical Sciences and Engineering, University of Maine, USA

There is a recipe for PBS with 2% TritonX 100 in the protocol above.
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garima tripathi Answered Nov 21, 2014

kims

PBS with 2 % trinton X100 menas? can you elaborate
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Michelle F Goody Answered Nov 21, 2014

Graduate School of Biomedical Sciences and Engineering, University of Maine, USA

We buy phalloidin from Life Technologies and dissolve it in 1.5 mL MeOH. We then aliquot and freeze the phalloidin stock at -20 C. Before incubating the zebrafish embryos in phalloidin, it is diluted 1 part phalloidin to 19 parts PBS with 2% Triton-X 100.
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