Hi Rumen,did you ever observe that parts of the tissue remains...

Dear Paula,

Recently, we had cases of blue staining remaining in the base of the root (next to the hypocotyl). This happened with plants that take up more iron and the overall iron content was strongly elevated.

I suppose the fixation and infiltration conditions might have to be optimized in this case but we have not tackled the problem yet. I will write back if we find a solution for this one.

Rumen