gel is coming out of the slide during lysing and electrophoresis....

protocol Protocol: Neutral Comet Assay
gel is coming out of the slide during lysing and electrophoresis. how to make it stick to the slide?
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nive thiagarajan Answered Dec 2, 2014

psg college of technology

Thank you so much. The cold temperature tip really helped :)
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Elisa Boutet-Robinet Author Answered Nov 24, 2014

Toxalim, Research Centre in Food Toxicology, INRA, Université de Toulouse, UPS, UMR1331, France

There are different critical steps about the gel (especially the points 3.2 to 3.8 of the on-line protocol) that can influence how it will stick during all the experiment. The first important thing is the temperature of the slide and of the buffers. Everything must be cold. The room temperature must also not be too high. The time (5 to 10 minutes) for which the slide stay on ice-pack and the temperature of the ice-pack is also important (take it out of the freezer 10-20 minutes before the use for the experiment). It is also important to slide off very gently the coverslip after the agarose has solidified.
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