For Calcium quantification, Do I have to add the decalcifying solution, 0.1 N HCL, on cultured cells after washing with DPBS? Can you tell me more or send me your decalcification protocol? The 12 hours incubation at what temperature? do i have to incubate it on a rotator orRead moreRead less
Department of Microbiology, Yeungnam University College of Medicine, Korea
To quantify calcium, cultured cells need to be washed twice with PBS and decalcified with 0.6 N HCl for 12 h under room temperature. You do not need to incubated cells on a rotator or shaker.