Hi,For Calcium quantification, Do I have to add the decalcifying...

protocol Protocol: In vitro Biomineralization Assay
Hi,

For Calcium quantification, Do I have to add the decalcifying solution, 0.1 N HCL, on cultured cells after washing with DPBS? Can you tell me more or send me your decalcification protocol? The 12 hours incubation at what temperature? do i have to incubate it on a rotator or
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Daewon Jeong Author Answered Nov 13, 2014

Department of Microbiology, Yeungnam University College of Medicine, Korea

To quantify calcium, cultured cells need to be washed twice with PBS and decalcified with 0.6 N HCl for 12 h under room temperature. You do not need to incubated cells on a rotator or shaker.
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