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Hi there,I ran this experiment and I managed to form tumorspheres....
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Colm Rowan Nov 5, 2014
Hi there,
I ran this experiment and I managed to form tumorspheres. However the tumorspheres seem to be adherent. I used ultra low attachment 96 well plates but the spheres don't seem to move under the microscope when the plate is moved? Has this happened to anyone else?
I ran this experiment and I managed to form tumorspheres. However the tumorspheres seem to be adherent. I used ultra low attachment 96 well plates but the spheres don't seem to move under the microscope when the plate is moved? Has this happened to anyone else?
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Sara Johnson Author Answered Nov 11, 2014
University of South Carolina
Hello,
I have had this happen once before with tumorspheres kept in culture for over one week. It is possible that the tumorspheres have remained in culture too long. We generally counted spheres after one week, so a lengthier culture can lead to attachment. From my understanding, some cells respond better to different types of non-tissue culture petri dishes and low attachment plates as well.
I hope this helps.
Sara
I have had this happen once before with tumorspheres kept in culture for over one week. It is possible that the tumorspheres have remained in culture too long. We generally counted spheres after one week, so a lengthier culture can lead to attachment. From my understanding, some cells respond better to different types of non-tissue culture petri dishes and low attachment plates as well.
I hope this helps.
Sara
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