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Hi,I am planning to do this assay for my research work. Could...
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Keshav Kumar Oct 6, 2014
Hi,
I am planning to do this assay for my research work. Could you please clarify my following questions?
1] Do I need to wash the plate bound CD3 before I seed the cells? I am confused with the step no 5. Why do I need to change the cells to new plate on day 2? Do I Read more
I am planning to do this assay for my research work. Could you please clarify my following questions?
1] Do I need to wash the plate bound CD3 before I seed the cells? I am confused with the step no 5. Why do I need to change the cells to new plate on day 2? Do I Read more
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Jia Li Author Answered Oct 12, 2014
SRI International
Hi,
1. Yes, wash the extra CD3. check the regular protocol for CD3 plate coating.
see my previous answer:"Because anti-CD3 will cause cell death, that's why anti-CD3 is plate-bound and we transfer culture to new plate."
check the protocol step 5" Transfer the culture to new plate after 2 days without digesting or changing the medium."
1. Yes, wash the extra CD3. check the regular protocol for CD3 plate coating.
see my previous answer:"Because anti-CD3 will cause cell death, that's why anti-CD3 is plate-bound and we transfer culture to new plate."
check the protocol step 5" Transfer the culture to new plate after 2 days without digesting or changing the medium."
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