Could I use the same protocol for RIP from Tissue...

Hi,

I only used cell lysates to get the target protein. You know the principle of this method is to use specificity of Ag-Ab reaction. If your target protein is outside of the cells, you should think about it amount is enough or not for this method. From theory, it is feasible. the problem is how do you extract the raw protein products. If you use other biochemical methods to purify the target protein , then run RIP, it may be better.