Improve Research Reproducibility A Bio-protocol resource

Hello Sir, really appreciate your post. I will like your assistance,...

Damilola Tanimowo Sep 12, 2014

Protocol: Analysis of Mouse Liver Glycogen Content

Hello Sir, really appreciate your post. I will like your assistance, i am to determine glycogen levels in poultry tissue, but standards are not available here and take between 6-8weeks for shipping after orders have been made. Using the PHESUL method is there a way that glucose could be us Read more more

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Peichuan Zhang Answered Jan 18, 2015

Department of Biology, The Pennsylvania State University, USA

Hi Damilola,

I have never done this type of assay. You may refer to other online protocols, for example, chromatography based
http://www.ncbi.nlm.nih.gov/pubmed/12560353

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P

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Damilola Tanimowo Answered Jan 14, 2015

University of Ibadan

Hello Sir, I would like to know is there a colorimetric determination protocol for betaine and dimethylglicine in blood?

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Peichuan Zhang Answered Sep 22, 2014

Department of Biology, The Pennsylvania State University, USA

Hi Damilola,

Actually, we used glucose (10ul, 0.5mM) as the standard for calculation in the assay. The acid hydrolysis step converts glycogen into free glucose, which then can be measured by the HK assay kit. You can find the assay kit from Sigma http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/gahk20bul.pdf

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P

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