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Preparation of L-cell conditioned medium: culture L929 cells...

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yu liming Sep 8, 2014
protocol Protocol: Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
Preparation of L-cell conditioned medium: culture L929 cells with initial 50% confluence in RPMI + 10% FBS for 5 days , but others use RPMI without FBS for 7 days, does it matter?
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Ran Chen Author Answered Sep 12, 2014

Zhejiang JFK Biological Technology Co. Ltd.

Never tested the condition without FBS. Not sure about this.
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