What is your recipe for the YES medium?

HE
Henry Elix
Sep 8, 2014
protocol Protocol: Yeast Vacuole Staining with FM4-64
What is your recipe for the YES medium?
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Bio-protocol Editorial Office Author Answered Sep 24, 2014

Bio-protocol


Hi Henry, you can find the recipe of the YES medium at https://www.atcc.org/~/media/3907D6168ED64037BCF4693AE75FBA2C.ashx
I also copied it below:

ATCC medium: 2064 YES Medium
Yeast extract................5.0 g
Glucose.....................30.0 g
L-Adenine..................225.0 mg
L-Histidine................225.0 mg
L-Leucine..................225.0 mg
L-Lysine HCl...............225.0 mg
Uracil.....................225.0 mg
Distilled water..............1.0
Sterilize medium by 0.2 micrometer filtration.
For solid medium:
Add 20.0 g agar to 500 ml distilled water and heat to boiling with
stirring to dissolve. Autoclave agar solution at 121C for 15 minutes.
Cool to 50C. Dissolve nutrient components in 500 ml distilled water and
filter-sterilize. Warm the nutrient solution to 50C. Aseptically combine
and mix the agar and nutrient solutions and dispense as required.
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