HOW TO USE PHYTAGEL IN MEDIUM BECAUSE I WANT SOLIDIFICATION...

mohammed dawood
mohammed dawood
Sep 6, 2014
protocol Protocol: Stable Transformation in Lotus japonicus
HOW TO USE PHYTAGEL IN MEDIUM BECAUSE I WANT SOLIDIFICATION MEDIUM ADJUST PH TO 4 BUT WHEN I USE AGARE MEDIUM CAN NOT BE SOLID SO I WANT SAK I ADD HPYTAGEI BEFORE AUTOCLAVING OR AFTER
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Takuya Suzaki Author Answered Sep 24, 2014

Division of Symbiotic Systems, National Institute for Basic Biology, Japan

Phytagel should be added the medium before autoclaving. The pH of the medium does not affect the solidification in this case.
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