Improve Research Reproducibility A Bio-protocol resource

I am trying to troubleshoot an EMSA and came across your protocol....

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Randi Foxall Sep 5, 2014
protocol Protocol: A Protocol for Electrophoretic Mobility Shift Assay (EMSA) from Primary Neuron
I am trying to troubleshoot an EMSA and came across your protocol. I am performing an EMSA using 5' biotin ssDNA, not dsDNA as in your protocol. Do you know if a ssDNA probe should work with the detection assays for Biotin?
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Jiali Li Author Answered Sep 6, 2014

Rutgers University

It should work too if it binds to your target efficiently.
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