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Hai, Thanks for this protocol. If I need to take the cytoplasmic...

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Charukesi Rajulu Aug 27, 2014
protocol Protocol: Nuclear Extraction from Arabidopsis thaliana
Hai,
Thanks for this protocol.
If I need to take the cytoplasmic fraction, at which step I should take the supernatent?
Thanks
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Charukesi Rajulu Answered Aug 28, 2014

JSS Academy of Higher Education & Research, Mysuru, Karnataka

Thanks a lot.
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Fang Xu Author Answered Aug 27, 2014

Shandong University

The supernatant after centrifugation in step 4 is the cytoplasmic fraction. You can transfer the supernatant carefully into a new container if you need all of them. Otherwise, you can take a small portion for analysis and dump the rest.
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